Elderberry (Sambucus nigra L.) Fruit Extract Alleviates Oxidative Stress, Insulin Resistance, and Inflammation in Hypertrophied 3T3-L1 Adipocytes and Activated RAW 264.7 Macrophages
| Autor: | Katarzyna Kowalska, Radosław Dembczyński, Joanna Zielińska-Wasielica, Anna Olejnik, Mariola Olkowicz |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
elderberry polyphenols medicine.medical_specialty obesity Health (social science) medicine.medical_treatment Adipose tissue Plant Science lcsh:Chemical technology medicine.disease_cause Health Professions (miscellaneous) Microbiology Intestinal absorption Article Proinflammatory cytokine functional food 03 medical and health sciences Insulin resistance Internal medicine medicine lcsh:TP1-1185 adipokines 030109 nutrition & dietetics Adiponectin Chemistry Insulin immune-metabolic effects 3T3-L1 medicine.disease glucose uptake 030104 developmental biology Endocrinology digestive enzymes fat cells Oxidative stress Food Science intracellular reactive oxygen species |
| Zdroj: | Foods Volume 8 Issue 8 Foods, Vol 8, Iss 8, p 326 (2019) |
| ISSN: | 2304-8158 |
| DOI: | 10.3390/foods8080326 |
| Popis: | Oxidative stress and inflammation in hypertrophied adipose tissue with excessive fat accumulation play a crucial role in the development of obesity and accompanying metabolic dysfunctions. This study demonstrated the capacity of elderberry fruit (EDB) extract to decrease the elevated production of reactive oxygen species in hypertrophied 3T3-L1 adipocytes. Treatment with the EDB extract resulted in modulation of mRNA expression and protein secretion of key adipokines in hypertrophied adipocytes. Expression of leptin and adiponectin was, respectively, down- and up-regulated. Moreover, glucose uptake stimulation was noticed in mature adipocytes, both sensitive to insulin and insulin resistant. This may suggest a positive effect of EDB extract on insulin resistance status. The extract was also found to alleviate the inflammatory response in activated RAW 264.7 macrophages by down-regulating the expression of proinflammatory genes (TNF-&alpha IL-6, COX-2, iNOS) and suppressing the enhanced production of inflammatory mediators (TNF-&alpha IL-6, PGE2, NO). In vitro experiments showed that the EDB extract could inhibit digestive enzymes, including &alpha amylase, &alpha glucosidase, and pancreatic lipase, leading to reduced intestinal absorption of dietary lipids and carbohydrates. Further in vivo studies could be postulated to support EDB as a functional food component for the prevention and treatment of obesity and metabolic-immune comorbidities. |
| Databáze: | OpenAIRE |
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