Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells
| Autor: | Yoshitaka Taketomi, Seigoh Takamatsu, Ichiro Kudo, Atsushi Ichikawa, Makoto Murakami, Takayuki Kainoh, Kazuyuki Furuta, Hirotsugu Takano, Naritoshi Shirata, Yukihiko Sugimoto, Kazuhisa Nakayama, Gozoh Tsujimoto, Yasunori Kozutsumi, Yuko Naito, Hiromu Takematsu, Sohken Tsuchiya, Satoshi Tanaka, Shunsuke Nakazawa, Yasushi Okuno |
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| Rok vydání: | 2008 |
| Předmět: |
Microarray
Biophysics Cell Culture Techniques Connective tissue Bone Marrow Cells Biology Biochemistry Models Biological Gi Mast cell Mice Structural Biology Gene expression Genetics medicine Animals Mast Cells Molecular Biology Cells Cultured Oligonucleotide Array Sequence Analysis Swiss 3T3 Cells Mice Inbred BALB C Gene Expression Profiling Cell Differentiation Cell Biology Cell biology Gene expression profiling Interleukin 33 medicine.anatomical_structure Cell culture Differentiation Immunology Female Peritoneum Histamine Peptide Hydrolases |
| Zdroj: | FEBS letters. 582(10) |
| ISSN: | 0014-5793 |
| Popis: | To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs. |
| Databáze: | OpenAIRE |
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