Bile-salt stimulated lipase polymorphisms do not associate with HCV susceptibility
| Autor: | Jan van der Meer, Richard Molenkamp, Kees Brinkman, Janke Schinkel, William A. Paxton, David Kwa, Gaby S. Steba, Sylvie M. Koekkoek, Maria Prins, Marc van der Valk, Georgios Pollakis |
|---|---|
| Přispěvatelé: | Graduate School, Medical Microbiology and Infection Prevention, AII - Infectious diseases, Infectious diseases, APH - Global Health, APH - Digital Health, APH - Personalized Medicine |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Cancer Research Sexual transmission Genotype Single-nucleotide polymorphism Biology 03 medical and health sciences Exon chemistry.chemical_compound Virology Gene expression Humans Homosexuality Male Substance Abuse Intravenous Gene Genetic Association Studies 030304 developmental biology Aged chemistry.chemical_classification 0303 health sciences 030306 microbiology Genetic Variation Exons Lipase Middle Aged Molecular biology Hepatitis C Infectious Diseases chemistry HIV-1 Glycoprotein DNA |
| Zdroj: | VIRUS RESEARCH Virus research, 274:197715. Elsevier |
| ISSN: | 1872-7492 0168-1702 |
| Popis: | Bile-salt stimulate lipase (BSSL) is a glycoprotein found in human milk and blood that can potently bind DC-SIGN. The BSSL gene is highly polymorphic with a variant number of O-linked glycosylated 11 amino acid repeats at the C-terminus of the protein, encoded in exon 11 of the gene. It has been shown that certain BSSL genotypes associate with decreased HIV-1 transmission in vitro and decreased HIV-1 disease progression. The protein forms dimers and individuals possessing one high (typically 14–21) and one low (typically 7–11) number of repeat domains has been shown to have stronger binding of BSSL to DC-SIGN and HIV-1 inhibitory activity in vitro. Since we previously demonstrated that SNPs within the DC-SIGN gene can associate with risk of HCV sexual transmission and which can be linked to diminished DC-SIGN gene expression we aimed to identify whether BSSL polymorphisms associated similarly through differential binding to DC-SIGN. DNA was isolated from the HIV-1 infected MSM cohort (MOSAIC) composed of HCV multiple exposed uninfected (MEU) (N = 30) and multiple exposed HCV infected (MEI) (N = 32) individuals and from the Amsterdam cohort studies (ACS) intravenous drug using (IDU) cohort (22 MEI and 40 MEU). The numbers of repeats in exon 11 were determined by PCR with repeat distributions compared between MEI and MEU. No statistical significant difference in the copy number of exon 11 repeats, or combinations of, in the BSSL gene was observed when comparing HCV infected MEI with MEU, thus the exon 11 repeat copy number in the BSSL gene does not affect HCV susceptibility. |
| Databáze: | OpenAIRE |
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