Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
| Autor: | Flávia Galindo Silvestre, Joab Trajano Silva, Ricardo Cezar, L.S. Fonseca, Flosi Paschoalin, Tavares Carvalho, Vânia Margaret |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Tuberculosis
Short Communication Veterinary Microbiology lcsh:QR1-502 multiplex-PCR Microbiology lcsh:Microbiology law.invention chemistry.chemical_compound law Multiplex polymerase chain reaction medicine bovine tuberculosis Pathogen Polymerase chain reaction Nose Mycobacterium bovis biology nasal swab biology.organism_classification medicine.disease Virology medicine.anatomical_structure chemistry Nasal Swab DNA |
| Zdroj: | Brazilian Journal of Microbiology Brazilian Journal of Microbiology, Vol 41, Iss 2, Pp 386-390 (2010) Brazilian Journal of Microbiology v.41 n.2 2010 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Volume: 41, Issue: 2, Pages: 386-390, Published: JUN 2010 |
| ISSN: | 1678-4405 1517-8382 |
| Popis: | Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested. |
| Databáze: | OpenAIRE |
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