Renal C3 synthesis in idiopathic membranous nephropathy: Correlation to urinary C5b-9 excretion
| Autor: | Raffaella Monno, Antonia Lopez, Carlo Manno, Valentina Cappiello, Vincenzo Montinaro, Francesco Paolo Schena, Loreto Gesualdo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Urinary system Fluorescent Antibody Technique Gene Expression Complement Membrane Attack Complex Kidney urologic and male genital diseases Glomerulonephritis Membranous Excretion chemistry.chemical_compound medicine Humans complement RNA Messenger urinary C5b-9 In Situ Hybridization Aged Creatinine Proteinuria medicine.diagnostic_test business.industry Complement C3 Middle Aged medicine.disease medicine.anatomical_structure chemistry Nephrology tubulointerstitial damage Mesangial proliferative glomerulonephritis Female Renal biopsy medicine.symptom membranous glomerulonephritis business Kidney disease |
| Zdroj: | Kidney International. 57(1):137-146 |
| ISSN: | 0085-2538 |
| DOI: | 10.1046/j.1523-1755.2000.00812.x |
| Popis: | Renal C3 synthesis in idiopathic membranous nephropathy: Correlation to urinary C5b-9 excretion Background Complement activation plays a central pathogenetic role in idiopathic membranous nephropathy (IMN). Urinary excretion of C5b-9 correlates to the immunologic activity of this disease. Recently, renal cortical C3 gene expression has been described in several nephropathies. Methods The aim of this study was to investigate the renal C3 gene expression by in situ hybridization in IMN and to correlate it with histopathologic, pathophysiologic, and immunologic (urinary C5b-9) indices of disease activity. Results C3 was expressed in 77% of 22 renal biopsies of IMN patients, mainly at the cortical tubular and glomerular parietal epithelial cell levels. C3 protein synthesis by tubular cells was demonstrated by immunofluorescence. The intensity of C3 gene expression by both glomerular and tubulointerstitial compartments correlated with the glomerular stage of disease ( P = 0.0023 and P = 0.0214, respectively). Although no correlation was found with proteinuria, serum creatinine at renal biopsy time was strongly associated with renal C3 expression. IMN patients showed a trend of increased urinary C5b-9 levels, which correlated to C3 at the tubulointerstitial level ( P = 0.0143). Conclusion Renal C3 production, mainly at the tubular level, may be induced by urinary excretion of C5b-9 in IMN and may have a pathogenetic role in the tubulointerstitial damage that can be associated with this disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|