Comparative Assessment of Cultures from Oral and Urethral Stem Cells for Urethral Regeneration

Autor: Graziella Pellegrini, Francesca Corradini, Giampaolo Bianchi, Michael Giovanardi, Massimo Lazzeri, Vicenzo G. Genna, Michela Zattoni, Anna Ribbene, Michele De Luca, Chiava Serafini, Guido Barbagli, F. Fidanza, Sofia Balò
Rok vydání: 2016
Předmět:
Keratinocytes
Pathology
medicine.medical_specialty
030232 urology & nephrology
Medicine (miscellaneous)
Biology
Stem cell marker
Epithelial cultures
Epithelial cultures
oral mucosa stem cells
tissue engineering
urethral regeneration
urethral stem cells
oral mucosa stem cells
Mice
03 medical and health sciences
urethral stem cells
0302 clinical medicine
Urethra
Single-cell analysis
Tissue engineering
medicine
Animals
Humans
Regeneration
Oral mucosa
Clonogenic assay
Cells
Cultured
Cell Proliferation
Cell Size
Polycomb Repressive Complex 1
Stem Cells
Tumor Suppressor Proteins
Regeneration (biology)
Mouth Mucosa
Epithelial Cells
3T3 Cells
General Medicine
urethral regeneration
Clone Cells
medicine.anatomical_structure
tissue engineering
030220 oncology & carcinogenesis
Single-Cell Analysis
Stem cell
Biomarkers
Transcription Factors
Zdroj: Current Stem Cell Research & Therapy. 11:643-651
ISSN: 1574-888X
DOI: 10.2174/1574888x10666150902094644
Popis: Urethral reconstruction has received much attention in recent years, due to pathologies such as recurrence of urethral strictures after treatments. Various surgical techniques have been developed to obtain the best risk-benefit ratio, such as autologous grafts taken from the oral cavity. Tissue engineering and stem cells, growing in a tissue from a small biopsies, can further improve surgery, reducing invasiveness and morbidity. To determine whether urethra or other epithelia can be equally useful for urethra engineering, a comparison of clonogenic ability, proliferative potential and stem cell markers should be obtained. In this study, 19 biopsies from urethra, and 21 from oral mucosa were obtained from patients, during reconstructive surgery. Urethral and oral tissues were removed from the same donor, to develop primary cultures and cell characterization. The long term regenerative properties of both tissues were investigated in vitro by life span, clonal analysis and markers of different clonal types. Results revealed the same high proliferative potential for urethra and oral mucosa cultures, but maintenance of specific markers. Karyotype and growth factor dependence confirmed the normal phenotype of cultured cells. Clonal analysis of the proliferative compartment highlighted a very different proportion of stem and transient amplifying cells, characterised by dissimilar cell size profile and marker expression. In conclusion, both tissues can be cultured and preserve their stem cells in vitro. Few differences appeared in oral mucosa vs urethra, suggesting that they can be equally useful for tissue engineering of the urethral tract.
Databáze: OpenAIRE
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