The effect of killer cell immunoglobulin-like receptor genotype on outcome of hematopoietic stem cell transplantation from matched sibling
| Autor: | Alaa Elhaddad, Ghada I. Mossallam, Sally Elfishawi, Raafat Abd El-Fattah, Azza M. Kamel |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Genotype medicine.medical_treatment Immunology Killer-cell immunoglobulin-like receptor chemical and pharmacologic phenomena Human leukocyte antigen Hematopoietic stem cell transplantation HLA-C Antigens Biology 03 medical and health sciences Young Adult 0302 clinical medicine Receptors KIR medicine Immunology and Allergy Humans Child Genotyping Polymorphism Genetic Histocompatibility Testing Siblings Haplotype Hematopoietic Stem Cell Transplantation General Medicine Middle Aged Transplantation Leukemia Myeloid Acute 030104 developmental biology Receptors KIR2DL5 Histocompatibility Female Transplantation Tolerance 030215 immunology KIR2DS4 |
| Zdroj: | Human immunology. 78(11-12) |
| ISSN: | 1879-1166 |
| Popis: | The alloreactivity of natural killer (NK) cell after allogeneic hematopoietic stem cell transplantation (AHSCT) is regulated by the interaction between donor killer immunoglobulin-like receptors (KIRs) and recipient human leukocyte antigen (HLA)-class I molecules. The aim was to identify KIR genes, haplotypes and their HLA-class I ligands and to investigate their association with transplantation outcome. The study included 65 patient/donor pairs who received AHSCT from HLA-matched identical siblings. KIR genotyping was done for donors using reverse sequence specific oligonucleotide probes (rSSO) coupled with luminex technology, while HLA-C genotyping was performed in patients using rSSO strip assay. In multivariate analysis, KIR2DS4 was associated with significant reduced incidence of relapse (p = .002). A trend towards reduced incidence of relapse was also observed with more than two KIR B motifs (p = .09), whereas a significant increased relapse was associated with homozygous HLA-C2 ligand compared to combined C1/C2 and C1/C1 (p = .04). Activating KIR2DS3 was associated with rapid leukocyte engraftment (p = .02). While, KIR 2DL5 was associated with decreased CMV infection (p = .03) and better platelets engraftment (p = .05). KIR genes, haplotypes and HLA-C alleles have an impact on HSCT outcome. Better selection of donors with favorable KIR genotype can improve HLA-matched sibling HSCT outcome especially for AML patients. |
| Databáze: | OpenAIRE |
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