Defining the roles of perforin, Fas/FasL, and tumour necrosis factor in T cell induced mucosal damage in the mouse intestine
| Autor: | Rajka Borojevic, Pengfei Zhou, Robert H. Riddell, R Maric, Michael Merger, Kenneth Croitoru, David A. Clark, Eckhard R. Podack, Darlene Steele-Norwood, Joanne L. Viney |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Fas Ligand Protein Necrosis CD3 Complex T-Lymphocytes T cell Apoptosis Mice SCID Biology Lymphocyte Activation Epithelium Receptors Tumor Necrosis Factor Fas ligand Interferon-gamma Mice Antigens CD Intestine Small In Situ Nick-End Labeling medicine Animals Receptors Tumor Necrosis Factor Type II Interferon gamma Intestinal Mucosa Receptor Mice Knockout Mice Inbred BALB C Membrane Glycoproteins Perforin Tumor Necrosis Factor-alpha Inflammation and Inflammatory Bowel Disease Gastroenterology Antibodies Monoclonal Mice Inbred C57BL medicine.anatomical_structure Receptors Tumor Necrosis Factor Type I Models Animal Immunology Cancer research biology.protein Tumor necrosis factor alpha medicine.symptom medicine.drug |
| Zdroj: | Gut. 51:155-163 |
| ISSN: | 0017-5749 |
| Popis: | Background and aims: Mucosal flattening and epithelial cell apoptosis are typical features of T cell induced inflammatory diseases of the bowel, such as coeliac disease and graft versus host disease. Mice injected with a T cell activating anti-CD3 antibody develop a severe diarrhoeal illness. We describe the histological features of this enteropathy and define the effector mechanisms involved in T cell induced mucosal injury in this in vivo model. Methods: Wild-type and genetically modified mice were injected with the anti-CD3 antibody 3C11 (50 μg). Changes in the murine intestine were characterised by light microscopy analysis and terminal uridine nick-end labelling (TUNEL) assay. The role of perforin, Fas/Fas ligand (FasL), tumour necrosis factor α (TNF-α), and interferon γ (IFN-γ) in T cell induced mucosal damage was assessed using selected immunodeficient mouse strains. Results: T cell activation caused severe damage, including small intestinal mucosal flattening and apoptosis of crypt epithelial cells. Mucosal damage was unaltered in anti-CD3 treated mice lacking IFN-γ, Fas, or TNF-α receptors. In mice lacking TNF-α receptors and Fas (TNF-R1×R2 lpr/lpr strain), enterocyte apoptosis was diminished but there was no significant reduction in tissue damage. Apoptosis and mucosal injury were significantly reduced in perforin knockout mice. Abrogation of both FasL and perforin (perforin KO×gld mice) further significantly reduced tissue damage and apoptotic bodies. Conclusions: T cell induced mucosal injury is mediated by the combined effect of multiple pathways but predominantly by perforin. The redundancy of the mechanisms of tissue damage will have significant impact on therapeutic strategies aimed at specific and targeted inhibition of inflammatory processes. |
| Databáze: | OpenAIRE |
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