Visualizing the Dynamics of T Cell–Dendritic Cell Interactions in Intact Lymph Nodes by Multiphoton Confocal Microscopy
| Autor: | Olena Kamenyeva, Ryan Kissinger, Juraj Kabat, Billur Akkaya |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell type Intravital Microscopy T-Lymphocytes T cell Cell Cell Communication Article law.invention Mice 03 medical and health sciences 0302 clinical medicine Antigen Cell Movement Confocal microscopy law medicine Animals Antigen Presentation Microscopy Confocal Chemistry Cell Differentiation Dendritic Cells Dendritic cell Cell biology Microscopy Fluorescence Multiphoton 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Lymph Nodes Lymph Immunocompetence Peripheral lymph Software |
| Zdroj: | Confocal Microscopy ISBN: 9781071614013 Methods Mol Biol |
| DOI: | 10.1007/978-1-0716-1402-0_13 |
| Popis: | Multiphoton microscopy has provided us the ability to visualize cell behavior and biology in intact organs due to its superiority in reaching deep into tissues. Because skin draining lymph nodes are readily accessible via minimal surgery, it is possible to characterize the intricate interactions taking place in peripheral lymph nodes intravitally. Here we describe our protocol to visualize antigen-specific T cell-dendritic cell interactions in the popliteal lymph node of immunocompetent mice. With this method, behaviors of up to four cell types, such as T cells with different antigen specificities, T cells differentiated into different effector and regulatory lineages and dendritic cells originating from mice that bear mutations in functional genes can be imaged simultaneously. |
| Databáze: | OpenAIRE |
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