miRNAs and neural alternative polyadenylation specify the virgin behavioral state
| Autor: | Elena Li, Daniel L. Garaulet, Lu Wei, Eric C. Lai, Binglong Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Untranslated region
Polyadenylation Mutant Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 0302 clinical medicine Gene expression microRNA medicine Animals Drosophila Proteins RNA Messenger Hox gene Molecular Biology Psychological repression 030304 developmental biology Homeodomain Proteins Neurons 0303 health sciences Mutation Behavior Animal Three prime untranslated region Cell Biology Phenotype Cell biology MicroRNAs Drosophila melanogaster Bithorax complex Female 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Dev Cell |
| DOI: | 10.1101/2020.04.05.026831 |
| Popis: | SummaryHow are diverse regulatory strategies integrated to impose appropriately patterned gene expression that underliein vivophenotypes? Here, we reveal how coordinated miRNA regulation and neural-specific alternative polyadenylation (APA) of a single locus controls complex behaviors. Our entry was the unexpected observation that deletion of Bithorax-Complex (BX-C) miRNAs converts virgin female flies into a subjective post-mated behavioral state, normally induced by seminal proteins following copulation. Strikingly, this behavioral switch is directly attributable to misregulation ofhomothorax(hth). We localize specific CNS abdominal neurons where de-repressed Hth compromises virgin behavior in BX-C miRNA mutants. Moreover, we use genome engineering to demonstrate that precise mutation ofhth3’UTR sites for BX-C miRNAs, or deletion of its neural 3’ UTR extension containing most of these sites, both induce post-mated behaviors in virgins. Thus, facilitation of miRNA-mediated repression by neural APA is required for virgin females to execute behaviors appropriate to their internal state. |
| Databáze: | OpenAIRE |
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