A recombinant protein based on Trypanosoma cruzi P21 enhances phagocytosis
| Autor: | Tatiana M. Clemente, Mario Cruz, Paula Cristina Brígido, Rafael G. B. Gomes, Adele A. Rodrigues, Eduardo Horjales Reboredo, Diana Bahia, Heline Hellen Teixeira Moreira, Claudio Vieira da Silva, Juliana Terzi Maricato, Fabrício Castro Machado, Luiz Mario Janini, Flávia Alves Martins, Paulo César Santos, Marlus Alves dos Santos, Renato A. Mortara |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Proteomics
Trypanosoma Receptors CXCR4 Phagocytosis Trypanosoma cruzi Immunology Protozoan Proteins lcsh:Medicine Plasma protein binding Biology Protozoology Biochemistry Microbiology law.invention Mice Model Organisms law parasitic diseases Molecular Cell Biology Animals Protein Interactions lcsh:Science G protein-coupled receptor Multidisciplinary lcsh:R Proteins biology.organism_classification Recombinant Proteins Cell biology Host-Pathogen Interaction Mice Inbred C57BL Infectious Diseases Recombinant DNA Parastic Protozoans Medicine lcsh:Q Research Article Protein Binding |
| Zdroj: | PLoS ONE, Vol 7, Iss 12, p e51384 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND: P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His(6)) on inflammatory macrophages during phagocytosis. FINDINGS: Our results showed that P21-His(6) acts as a phagocytosis inducer by binding to CXCR4 chemokine receptor and activating actin polymerization in a way dependent onthe PI3-kinase signaling pathway. CONCLUSIONS: Thus, our results shed light on the notion that native P21 is a component related to T. cruzi evasion from the immune response and that CXCR4 may be involved in phagocytosis. P21-His(6) represents an important experimental control tool to study phagocytosis signaling pathways of different intracellular parasites and particles. |
| Databáze: | OpenAIRE |
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