Cloning and characterization of an aminoglycoside 6'-N-acetyltransferase gene from Citrobacter freundii which confers an altered resistance profile
| Autor: | Roberta S. Hare, George H. Miller, M G Blanco, K J Shaw, H Y Wu |
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| Rok vydání: | 1997 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Microbial Sensitivity Tests Integron Polymerase Chain Reaction Homology (biology) Plasmid Acetyltransferases Gene expression medicine heterocyclic compounds Pharmacology (medical) Amino Acid Sequence Cloning Molecular Gene Pharmacology Genetics Base Sequence biology Drug Resistance Microbial Kanamycin Sequence Analysis DNA biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Molecular biology Anti-Bacterial Agents Citrobacter freundii carbohydrates (lipids) Aminoglycosides Infectious Diseases biology.protein bacteria Research Article Plasmids medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.41.11.2439 |
| Popis: | A novel gene encoding a 6'-N-aminoglycoside acetyltransferase, aac(6')-In, has been cloned and sequenced from Citrobacter freundii 13996-19, a clinical isolate from Venezuela. This gene mediates resistance to amikacin, 2'-N-ethylnetilmicin, isepamicin, kanamycin, netilmicin, and tobramycin. The aac(6')-In gene is 573 nucleotides in length and encodes a putative protein of 190 amino acids. AAC(6')-In is most closely related to AAC(6')-Im and AAC(6')-Ie, demonstrating 64.4% and 62.3% similarity, respectively, at the protein level, suggesting these proteins share a common ancestor. The aac(6')-In flanking sequences demonstrated homology to integron- and transposon-related elements which are often found associated with resistance determinants. Hybridization studies performed with an intragenic probe specific for aac(6')-In indicate that this gene is prevalent within Venezuela but has not been observed outside of the country. Furthermore, the aac(6)-In gene was found in 10 different species of gram-negative bacteria. |
| Databáze: | OpenAIRE |
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