Identification of a Short Viral Transcript in Leishmania RNA Virus-infected Cells
| Autor: | In Kwon Chung, Jean L. Patterson, Timothy Currie Armstrong |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Base Sequence
Transcription Genetic biology Molecular Sequence Data RNA-dependent RNA polymerase RNA RNA virus DNA-Directed RNA Polymerases Blotting Northern biology.organism_classification Polymerase Chain Reaction Virology Molecular biology Reverse transcriptase Transcription (biology) RNA editing RNA polymerase I biology.protein Animals RNA Viruses RNA Messenger Leishmania guyanensis Polymerase RNA Double-Stranded |
| Zdroj: | Virology. 198:552-556 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1994.1066 |
| Popis: | Certain strains of Leishmania guyanensis carry persistently infecting double-stranded RNA viruses. The viral polymerase has been shown to have both transcriptase and replicase activity. To date, only full-length RNA transcription of minus and plus strands have been reported. This report describes the synthesis of a 320 nt transcript which is complementary to the 3' end of the minus strand RNA. This plus-stranded short transcript was first detected in an in vitro polymerase assay. It was also detected in virus-infected Leishmania cells by a reverse transcription-polymerase chain reaction assay and by Northern analysis of infected Leishmania cell RNA. |
| Databáze: | OpenAIRE |
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