Decreased expression of the DBC2 gene and its clinicopathological significance in breast cancer: correlation with aberrant DNA methylation
| Autor: | Jinlian Song, Lin Hou, Li Wu, Yinlin Ge, Zhongliang Ma, Dong-Liang Lin, Lin-Lin Han |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Gene Expression
CA 15-3 Breast Neoplasms Bioengineering Biology Real-Time Polymerase Chain Reaction Applied Microbiology and Biotechnology Breast cancer GTP-Binding Proteins Cell Line Tumor Gene expression medicine Humans skin and connective tissue diseases Gene Genetics Gene Expression Profiling Tumor Suppressor Proteins General Medicine Methylation DNA Methylation medicine.disease Gene expression profiling Real-time polymerase chain reaction DNA methylation Cancer research Female Biotechnology |
| Zdroj: | Biotechnology Letters. 35:1175-1181 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-013-1190-x |
| Popis: | Loss of DBC2 (deleted in breast cancer 2) gene expression is frequent in breast cancer tissues. This can be explained by homozygous deletions or other mutations in a minority of cases but alternative mechanisms need to be investigated. Here, DBC2 expression was significantly suppressed compared with normal breast tissues in breast cancer tissues when analyzed by RT-PCR. Furthermore, DNA methylation on DBC2 was more prevalent in breast tumors than in normal tissues. DBC2 mRNA levels correlated with the degree of DBC2 methylation in breast cancer tissues and in a breast cancer cell line (T47D). Clinico-pathological correlation analysis showed that DBC2 promoter methylation was associated with tumor-node-metastasis stages II and III/IV, lymph node metastasis, p53 mutation, and HER2-positive status. Thus loss of DBC2 expression is caused by abnormal methylation of DBC2 and might have a role in breast cancer development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink