Disease-free monoculture farming by fungus-growing termites
| Autor: | Silas Anselm Rasmussen, Michael Poulsen, Victoria L. Challinor, Duur K. Aanen, Christine Beemelmanns, Saria Otani, Sara Kildgaard, Nina B. Kreuzenbeck, Louise Lee Munk Larsen, Søren Krath Christensen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
animal structures lcsh:Medicine Isoptera Microbial Sensitivity Tests Fungus Evolutionary ecology Laboratorium voor Erfelijkheidsleer Article 03 medical and health sciences 0302 clinical medicine Anti-Infective Agents SDG 3 - Good Health and Well-being Termitomyces Symbiosis Botany Metabolomics Animals Life Science Internal transcribed spacer lcsh:Science Heterokaryon Life Cycle Stages Principal Component Analysis Multidisciplinary biology Obligate lcsh:R fungi PE&RC biology.organism_classification 030104 developmental biology Pyrosequencing lcsh:Q Laboratory of Genetics Metagenomics 030217 neurology & neurosurgery Bacteria |
| Zdroj: | Scientific Reports 9 (2019) 1 Scientific Reports, 9(1) Otani, S, Challinor, V L, Kreuzenbeck, N B, Kildgaard, S, Christensen, S K, Larsen, L L M, Aanen, D K, Rasmussen, S A, Beemelmanns, C & Poulsen, M 2019, ' Disease-free monoculture farming by fungus-growing termites ', Scientific Reports, vol. 9, 8819, pp. 1-10 . https://doi.org/10.1038/s41598-019-45364-z Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) Otani, S, Challinor, V L, Kreuzenbeck, N B, Kildgaard, S, Krath Christensen, S, Larsen, L L M, Aanen, D K, Rasmussen, S A, Beemelmanns, C & Poulsen, M 2019, ' Disease-free monoculture farming by fungus-growing termites ', Scientific Reports, vol. 9, no. 1, 8819 . https://doi.org/10.1038/s41598-019-45364-z Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Fungus-growing termites engage in an obligate mutualistic relationship with Termitomyces fungi, which they maintain in monocultures on specialised fungus comb structures, without apparent problems with infectious diseases. While other fungi have been reported in the symbiosis, detailed comb fungal community analyses have been lacking. Here we use culture-dependent and -independent methods to characterise fungus comb mycobiotas from three fungus-growing termite species (two genera). Internal Transcribed Spacer (ITS) gene analyses using 454 pyrosequencing and Illumina MiSeq showed that non-Termitomyces fungi were essentially absent in fungus combs, and that Termitomyces fungal crops are maintained in monocultures as heterokaryons with two or three abundant ITS variants in a single fungal strain. To explore whether the essential absence of other fungi within fungus combs is potentially due to the production of antifungal metabolites by Termitomyces or comb bacteria, we performed in vitro assays and found that both Termitomyces and chemical extracts of fungus comb material can inhibit potential fungal antagonists. Chemical analyses of fungus comb material point to a highly complex metabolome, including compounds with the potential to play roles in mediating these contaminant-free farming conditions in the termite symbiosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|