Attempts to Separate Female Ascaris suum Antigen and to Investigate its Partial Characterization
| Autor: | K. Okamoto, S Nagai, M Takashima, T. Watanabe, H Ohmi, M. Kanoe |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
Sex Determination Analysis Swine Sodium Immunoblotting chemistry.chemical_element Serology chemistry.chemical_compound Antigen Animals Ammonium Ascaris suum Polyacrylamide gel electrophoresis Specific-pathogen-free Swine Diseases chemistry.chemical_classification General Veterinary biology Ovary biology.organism_classification Molecular biology chemistry Antigens Helminth Electrophoresis Polyacrylamide Gel Female Animal Science and Zoology Glycoprotein |
| Zdroj: | The Veterinary Journal. 165:164-168 |
| ISSN: | 1090-0233 |
| DOI: | 10.1016/s1090-0233(02)00166-1 |
| Popis: | The location and separation of Ascaris suum antigen for serological testing was investigated. The antigenic constituent was rich in the ovary of the adult worm and was obtained by dialysis with 50% ammonium sulphate saturated solution. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting analysis demonstrated that the heat labile antigenic preparation showed one major and seven faint bands. The major band seemed also to be a glycoprotein. The sera from pigs with/without hepatic milk spot showed relatively high precipitation titres, while, those from the specific pathogen free pigs manifested low titres. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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