A simple and static preservation system for shipping retinal pigment epithelium cell sheets
| Autor: | Naoshi Koide, Junichi Kuwabara, Masayo Takahashi, Kanji Hori, Mitsuhiro Nishida, Yuji Tanaka |
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| Rok vydání: | 2018 |
| Předmět: |
Computer science
Mrna expression 0206 medical engineering Preservation Biological Biomedical Engineering Medicine (miscellaneous) Transportation 02 engineering and technology Retinal pigment epithelium cell Retinal Pigment Epithelium Regenerative medicine Patient care Cell Line Biomaterials 03 medical and health sciences medicine Humans Cell sheet 030304 developmental biology 0303 health sciences Retinal pigment epithelium Temperature Reproducibility of Results Epithelial Cells 020601 biomedical engineering Bench to bedside Tissue transfer Culture Media medicine.anatomical_structure Stress Mechanical Rheology Biomedical engineering |
| Zdroj: | Journal of tissue engineering and regenerative medicine. 13(3) |
| ISSN: | 1932-7005 |
| Popis: | The ability to move cells and tissues from bench to bedside is an essential aspect of regenerative medicine. In this study, we propose a simple and static shipping system to deliver tissue-engineered cell sheets. Notably, this system is electronic-device-free and simplified to minimize the number of packing and opening steps involved. Shipping conditions were optimized, and application and verification of the system were performed using human iPS cell-derived or fetal retinal pigment epithelium (RPE) cell sheets. The temperature of the compartments within the insulated container was stable at various conditions, and filling up the cell vessel with medium effectively prevented turbulence-induced mechanical damage to the RPE cell sheets. Furthermore, no abnormal changes were observed in RPE morphology, transepithelial electrical resistance, or mRNA expression after transit by train and car. Taken together, our simple shipping system has the potential to minimize the costs and human error associated with bench to bedside tissue transfer. This specially designed regenerative tissue shipping system, validated for use in this field, can be used without any special training. This study provides a procedure for easily sharing engineered tissues with the goal of promoting collaboration between laboratories and hospitals and enhancing patient care. |
| Databáze: | OpenAIRE |
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