Cross Talk Between p22phox and ATF4 in the Endothelial Unfolded Protein Response
| Autor: | Zuwen Zhang, Andreas Petry, Benjamin Trautz, Agnes Görlach, Florian Rieß |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Physiology Clinical Biochemistry Endoplasmic Reticulum Biochemistry 03 medical and health sciences Humans Molecular Biology Cells Cultured General Environmental Science chemistry.chemical_classification Reactive oxygen species 030102 biochemistry & molecular biology biology Endoplasmic reticulum ATF4 Endothelial Cells NADPH Oxidases Cell Biology Endoplasmic Reticulum Stress Activating Transcription Factor 4 Cell biology 030104 developmental biology chemistry biological sciences Unfolded Protein Response biology.protein Unfolded protein response General Earth and Planetary Sciences P22phox HeLa Cells |
| Zdroj: | Antioxidants & Redox Signaling. 30:40-55 |
| ISSN: | 1557-7716 1523-0864 |
| Popis: | Cardiovascular diseases have been associated with stress in the endoplasmic reticulum (ER) and accumulation of unfolded proteins leading to the unfolded protein response (UPR). Reactive oxygen species (ROS) such as superoxide and HIn this study, we investigated the role of p22phox, an essential component of most NADPH oxidases, in the UPR of endothelial cells.Induction of ER stress increased p22phox expression at the transcriptional level. p22phox was identified as novel target of the UPR transcription factor ATF4 (activator of transcription factor 4) under ER stress conditions by promoter analyses and ChIP. Depletion of ATF4 and p22phox diminished the levels of superoxide and Hp22phox is a novel target of ATF4 in response to ER stress, which can promote the PERK-ATF4 branch of the UPR in vitro and in vivo.p22phox-dependent NADPH oxidases are important mediators of ER stress driving the UPR. |
| Databáze: | OpenAIRE |
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