Emergence of a novel recombinant of CV-A5 in HFMD epidemics in Xiangyang, China
| Autor: | Jia Lu, Shuo Shen, Xuhua Guan, Yeqing Tong, Shengli Meng, Zhen-Ni Wei, Jian-Yi Mai, Jin Weiping, Zhi-Yu Luo, Zejun Wang, Yu-Ting Yu, Qian Shasha |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Serotype
China Coxsakievirus A5 Biology QH426-470 medicine.disease_cause law.invention law Genotype medicine Genetics Humans Epidemics Genotyping Internal medicine Phylogeny Genetics (clinical) Polymerase chain reaction Enterovirus Phylogenetic tree Foot-and-mouth disease Hand foot and mouse disease Outbreak medicine.disease Virology RC31-1245 Recombinantion Hand Foot and Mouth Disease Research Article |
| Zdroj: | BMC Medical Genomics, Vol 14, Iss 1, Pp 1-9 (2021) BMC Medical Genomics |
| ISSN: | 1755-8794 |
| Popis: | Background Hand, foot and mouth disease (HFMD) is caused by a variety of enterovirus serotypes and the etiological spectrum worldwide has changed since a large scale of outbreaks occurred in 1997. Methods A large number of clinical specimens of HFMD patients were collected in Xiangyang and genotyping was performed by qRT-PCR, conventional PCR amplification and sequencing. Among the 146 CV-A5 detected cases, the complete genome sequences of representative strains were determined for genotyping and for recombination analysis. Results It was found that CV-A5 was one of the six major serotypes that caused the epidemic from October 2016 to December 2017. Phylogenetic analyses based on the VP1 sequences showed that these CV-A5 belonged to the genotype D which dominantly circulated in China. Recombination occurred between the CV-A5 and CV-A2 strains with a breakpoint in the 2A region at the nucleotide 3791. Conclusions The result may explain the emergence of CV-A5 as one of the major pathogens of HFMD. A multivalent vaccine against HFMD is urgently needed to control the disease and to prevent emerging and spreading of new recombinants. |
| Databáze: | OpenAIRE |
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