Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
| Autor: | Laura Fernández, Alicia Margarita Godeas, Josefina Bompadre, Vanesa Analia Silvani, Mariana Pergola |
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| Rok vydání: | 2009 |
| Předmět: |
Hypha
biology Physiology GLOMUS INTRARADICES fungi MONOXENIC CULTURE TRANSFORMED SOYBEAN ROOTS General Medicine Genetically modified crops Fungus biology.organism_classification Applied Microbiology and Biotechnology Spore Ciencias Biológicas Glomeromycota Biología Celular Microbiología Symbiosis Botany Colonization Micología Mycorrhiza CIENCIAS NATURALES Y EXACTAS Biotechnology |
| Zdroj: | World Journal of Microbiology and Biotechnology. 25:1857-1863 |
| ISSN: | 1573-0972 0959-3993 |
| DOI: | 10.1007/s11274-009-0065-8 |
| Popis: | Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made between TSR system and plants of the same genotype. The extraradical fungal structures generated in vitro culture showed normal development. Straight runner hyphae branched into short simple branched absorbing structures and spores were initiated. AM symbiosis was confirmed by the presence of arbuscules and vesicles in cortical cells of the TSRs. The frequency of intraradical colonization in TSRs was higher than in plants grown in soil, whereas the intensity values of intraradical colonization in TSR cultures were similar to those in whole plants. These results show that TSR cultures were able to support the growth and characteristic development of G. intraradices. Fil: Fernández, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Silvani, Vanesa Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Bompadre, Maria Josefina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pergola, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Godeas, Alicia Margarita. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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