Nanometer-scale physically adsorbed thermoresponsive films for cell culture
| Autor: | Yury Rochev, Deirdre Healy, Julia Madden, Alexander V. Gorelov, Peter Dockery, Kerry Thompson, Serguei Belochapkine, Maria E. Nash |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Materials science Polymers and Plastics General Chemical Engineering Nanotechnology 02 engineering and technology Polymer 021001 nanoscience & nanotechnology Lower critical solution temperature Analytical Chemistry 03 medical and health sciences 030104 developmental biology Adsorption chemistry Chemical engineering Cell culture Nanometre 0210 nano-technology |
| DOI: | 10.6084/m9.figshare.3493442 |
| Popis: | Physical adsorption was used to produce nanometer thick thermoresponsive films with a view to nonenzymatic cell detachment. Two polymers were investigated, poly-(N-isopropylacrylamide) and poly (N-isopropylacrylamide-co-N-tertbutylacrylamide). Substrates were prepared above and below the polymers’ LCST to investigate the effect of polymer conformation on the prepared substrates. Endothelial cells were seeded on the prepared films; cell proliferation was higher on the films produced below the polymers’ LCST than on those prepared above and cells detached from the surfaces upon temperature reduction. Physical adsorption of poly-(N-isopropylacrylamide)–based films is a viable approach to produce substrates compliant with cell growth and temperature modulated detachment. |
| Databáze: | OpenAIRE |
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