Polyphosphatase PPN1 of Saccharomyces cerevisiae: Switching of Exopolyphosphatase and Endopolyphosphatase Activities
| Autor: | M. A. Eldarov, L. V. Trilisenko, Tatiana Kulakovskaya, Nadezhda A Andreeva |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Multidisciplinary
Saccharomyces cerevisiae Proteins Arginine Polyphosphate Science Saccharomyces cerevisiae Endopolyphosphatase activity Vacuole Biology Phosphate biology.organism_classification Acid Anhydride Hydrolases chemistry.chemical_compound Biochemistry chemistry Medicine Endopolyphosphatase Exopolyphosphatase Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 3, p e0119594 (2015) |
| ISSN: | 1932-6203 |
| Popis: | The polyphosphatase PPN1 of Saccharomyces cerevisiae shows an exopolyphosphatase activity splitting phosphate from chain end and an endopolyphosphatase activity fragmenting high molecular inorganic polyphosphates into shorter polymers. We revealed the compounds switching these activities of PPN1. Phosphate release and fragmentation of high molecular polyphosphate prevailed in the presence of Co2+ and Mg2+, respectively. Phosphate release and polyphosphate chain shortening in the presence of Co2+ were inhibited by ADP but not affected by ATP and argininе. The polyphosphate chain shortening in the presence of Mg2+ was activated by ADP and arginine but inhibited by ATP. |
| Databáze: | OpenAIRE |
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