The use of neutral and non-neutral SSRs to analyse the genetic structure of a Tunisian collection of Medicago truncatula lines and to reveal associations with eco-environmental variables
| Autor: | G. Cardinet, Thierry Huguet, Mohamed Elarbi Aouani, F. Lazrek, J. Ronfort, Fabien Chardon, Valérie Roussel |
|---|---|
| Přispěvatelé: | Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Centre de Biotechnologie de Borj Cedria (CBBC), École nationale supérieure agronomique de Toulouse [ENSAT], Diversité et adaptation des plantes cultivées (UMR DIAPC), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
0106 biological sciences
Tunisia DNA Plant Genotype GENETICS COLLECTIONS [SDV]Life Sciences [q-bio] Population Population genetics Plant Science Environment Balancing selection NONNEUTRAL SSRS 01 natural sciences POPULATIONS NATURELLES 03 medical and health sciences Medicago truncatula ALLELES NON NEUTRES education NEUTRAL SSRS 030304 developmental biology Local adaptation 0303 health sciences Genetic diversity education.field_of_study BIOLOGIE DES POPULATIONS biology Ecology NATURAL POPULATIONS food and beverages General Medicine GENETIQUE biology.organism_classification GENOTYPES Genetics Population Insect Science Genetic structure Microsatellite Animal Science and Zoology BIODIVERSITY Genome Plant Microsatellite Repeats 010606 plant biology & botany |
| Zdroj: | Genetica Genetica, Springer Verlag, 2009, 135 (3), pp.391-402. ⟨10.1007/s10709-008-9285-3⟩ |
| ISSN: | 0016-6707 1573-6857 |
| DOI: | 10.1007/s10709-008-9285-3⟩ |
| Popis: | Contact :thierry.huguet@ensat.fr; International audience; In this study, we investigated the genetic diversity of a collection of 136 Medicago truncatula lines from 10 Tunisian natural populations collected in well-defined locations and in various ecological conditions of soil, salinity and water availability. The genetic diversity was evaluated using a set of 18 microsatellites (SSRs), representing the 8 chromosomes of M. truncatula. A neutrality test showed that 7 SSRs were non-neutral with evidence of balancing selection. The 11 neutral SSRs revealed a geographical pooling with the Tunisian Dorsale axis restricting migration of alleles. The 7 non-neutral alleles demonstrate a correlation with rainfall, altitude and salinity environmental variables suggesting that these SSRs are linked to genes involved in water use efficiency, resistance to salinity or adaptation to altitude, and that there is local adaptation of M. truncatula to these variables. This demonstrates that the choice of so-called neutral markers should be carefully evaluated in population genetic studies. This study illustrates the genetic diversity occurring in natural Tunisian populations of M. truncatula and describes the first collection of this species dedicated to natural variation involved in adaptation to the environment |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink