Genomic profiling of the transcription factor Zfp148 and its impact on the p53 pathway
| Autor: | Ella M. Eklund, Jozefina J. Dzanan, Markus Lindberg, Anna Staffas, Ben O. Titmuss, Angana A. H. Patel, Erik Larsson, Per Lindahl, Zhiyuan V. Zou, Eva Forssell-Aronsson, Kristell Le Gal, Inger Johansson, Nadia Gul, Åsa Tivesten, Abdulmalik A. Bokhari, Volkan I. Sayin, Viktor Garellick, Martin O. Bergo |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Chromatin Immunoprecipitation Cell division lcsh:Medicine Down-Regulation Cell Cycle Proteins Biology Senescence Article Cell Line 03 medical and health sciences Mice 0302 clinical medicine Transcription (biology) RNA interference Animals RNA Small Interfering lcsh:Science Transcription factor Cyclin-Dependent Kinase Inhibitor p16 Etoposide Multidisciplinary Cell growth Activator (genetics) lcsh:R Promoter Cell Cycle Checkpoints Fibroblasts Cell Cycle Gene Cell biology E2F Transcription Factors Computational biology and bioinformatics DNA-Binding Proteins 030104 developmental biology Gene Ontology Gene Expression Regulation lcsh:Q RNA Interference CRISPR-Cas Systems Cisplatin Tumor Suppressor Protein p53 Chromatin immunoprecipitation 030217 neurology & neurosurgery DNA Damage Signal Transduction Transcription Factors |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Recent data suggest that the transcription factor Zfp148 represses activation of the tumor suppressor p53 in mice and that therapeutic targeting of the human orthologue ZNF148 could activate the p53 pathway without causing detrimental side effects. We have previously shown that Zfp148 deficiency promotes p53-dependent proliferation arrest of mouse embryonic fibroblasts (MEFs), but the underlying mechanism is not clear. Here, we showed that Zfp148 deficiency downregulated cell cycle genes in MEFs in a p53-dependent manner. Proliferation arrest of Zfp148-deficient cells required increased expression of ARF, a potent activator of the p53 pathway. Chromatin immunoprecipitation showed that Zfp148 bound to the ARF promoter, suggesting that Zfp148 represses ARF transcription. However, Zfp148 preferentially bound to promoters of other transcription factors, indicating that deletion of Zfp148 may have pleiotropic effects that activate ARF and p53 indirectly. In line with this, we found no evidence of genetic interaction between TP53 and ZNF148 in CRISPR and siRNA screen data from hundreds of human cancer cell lines. We conclude that Zfp148 deficiency, by increasing ARF transcription, downregulates cell cycle genes and cell proliferation in a p53-dependent manner. However, the lack of genetic interaction between ZNF148 and TP53 in human cancer cells suggests that therapeutic targeting of ZNF148 may not increase p53 activity in humans. |
| Databáze: | OpenAIRE |
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