Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress
| Autor: | Keita Suwabe, Hikaru Nanjo, Masao Watanabe, Kotomi Fujita, Moe Nabemoto, Masaaki Kobayashi, Satomi Sakazono, Kaori Yamamura, Mizuho Kawagishi, Tomoe Shindo, Toru Kudo, Shunsuke Maeda, Masaaki Osaka, Takashi Endo, Kuniaki Nagano, Kana Ito, Midori Taguchi, Go Suzuki, Kenichi Saeki, Hiromi Masuko-Suzuki, Kentaro Yano |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Regulation of gene expression Tapetum Abiotic stress Stamen food and beverages Oryza Flowers General Medicine Biology Cold Temperature MicroRNAs 03 medical and health sciences 030104 developmental biology Microspore Gene Expression Regulation Plant Stress Physiological Botany Gene expression Genetics Pollen Molecular Biology Gene Illumina dye sequencing |
| Zdroj: | Genes & Genetic Systems. 91:97-109 |
| ISSN: | 1880-5779 1341-7568 |
| DOI: | 10.1266/ggs.15-00056 |
| Popis: | Plants subjected to abiotic stress can regulate gene expression post-transcriptionally by means of small RNAs such as microRNAs. Cool-temperature stress causes abnormal tapetum hypertrophy in rice anthers, leading to pollen sterility. As a first step toward understanding the molecular mechanisms of cool tolerance in developing anthers of rice, we report here a comprehensive comparative analysis of microRNAs between cool-sensitive Sasanishiki and cool-tolerant Hitomebore cultivars. High-throughput Illumina sequencing revealed 241 known and 46 novel microRNAs. Interestingly, 15 of these microRNAs accumulated differentially in the two cultivars at the uninucleate microspore stage under cool conditions. Inverse correlations between expression patterns of microRNAs and their target genes were confirmed by quantitative RT-PCR analysis, and cleavage sites of some of the target genes were determined by 5' RNA ligase-mediated RACE experiments. Thus, our data are useful resources to elucidate microRNA-mediated mechanism(s) of cool tolerance in rice anthers at the booting stage. |
| Databáze: | OpenAIRE |
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