Isolation and profiling of plasma microRNAs: Biomarkers for asthma and allergic rhinitis
| Autor: | Faoud T. Ishmael, Kristin Lambert, Ronaldo P. Panganiban, Zoe Laryea, Man Hsun Hsu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Circulating mirnas
Inflammatory lung disease business.industry Disease medicine.disease Polymerase Chain Reaction Rhinitis Allergic Asthma General Biochemistry Genetics and Molecular Biology Gene expression profiling MicroRNAs Real-time polymerase chain reaction Immunology microRNA medicine Humans Biomarker (medicine) business Molecular Biology Biomarkers |
| Zdroj: | Methods. 152:48-54 |
| ISSN: | 1046-2023 |
| Popis: | Chronic inflammatory diseases can be particularly challenging to diagnose and characterize, as inflammatory changes in tissue may not be present in blood. There is a crucial need to develop non-invasive biomarkers that would be useful in diagnosing disease and selecting medical therapies. For example, there are no blood tests to diagnose asthma, a common inflammatory lung disease. MicroRNA (miRNA) expression profiling in blood is emerging as a potentially sensitive and useful biomarker of many diseases. In particular, we have characterized a cost-effective PCR-based array technology to measure and profile circulating miRNAs in the plasma of patients with allergic rhinitis and asthma. Here, we describe the methods to isolate, quantify, and analyze miRNAs in the plasma of human subjects as well as ways to determine their diagnostic utility. |
| Databáze: | OpenAIRE |
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