Effects of estrogens on proliferation and differentiation of neural stem/progenitor cells
| Autor: | Yoshiko Furukawa, Koichi Murase, Mitsunari Nakajima, Shoei Furukawa, Makiko Okada, Teppei Kaku, Akihisa Makino |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Telencephalon
medicine.medical_specialty medicine.medical_treatment Basic fibroblast growth factor Biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Phenols Internal medicine medicine Animals Progenitor cell Benzhydryl Compounds Rats Wistar Cells Cultured Cell Proliferation DNA Primers Base Sequence Estradiol Reverse Transcriptase Polymerase Chain Reaction Growth factor Stem Cells Neurogenesis Cell Differentiation General Medicine Embryonic stem cell Immunohistochemistry humanities Oligodendrocyte Cell biology Rats Endocrinology medicine.anatomical_structure chemistry Mitogen-activated protein kinase biology.protein Platelet-derived growth factor receptor |
| Zdroj: | Biomedical research (Tokyo, Japan). 29(3) |
| ISSN: | 1880-313X |
| Popis: | We investigated the effect of the female hormone 17beta-estradiol (E2) and the hormone mimic bisphenol A (BPA) on the proliferation and differentiation of rat neural stem/progenitors cells (NS/PCs) cultured from the telencephalon of embryonic day-15 rats. Basic fibroblast growth factor (FGF-2) is a potent mitogen of early generated NS/PCs, and is used for the proliferation of NS/PCs in vitro. Administration of E2 or BPA alone to the NS/PCs stimulated their proliferation in the absence but not in the presence of FGF-2. E2- or BPA-treatment increased the ratio of the oligodendrocytes generated from the NS/PCs to total cells; however, this ratio did not change when the cells were stimulated with platelet-derived growth factor (PDGF), a mitogen for oligodendrocyte precursors, or with neurotrophin-3, an oligogenic factor for glial progenitor cells. These results suggest that estrogens would influence the fate of NS/PCs when the cells are poorly supplied with mitogens or differentiation factors during the early stages of neurogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|