A comparison of protein S-thiolation (protein mixed-disulfide formation) in heart cells treated with t-butyl hydroperoxide or diamide
| Autor: | Mark W. Collison, James A. Thomas, Linda M. Grimm, Daniel Beidler |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Cell
Muscle Proteins Oxidative phosphorylation In Vitro Techniques Protein S chemistry.chemical_compound tert-Butylhydroperoxide medicine Animals Cysteine Disulfides Molecular Biology Cells Cultured Diamide biology Molecular mass Chemistry Cell Biology Metabolism Glutathione Myocardial Contraction Peroxides Rats medicine.anatomical_structure Biochemistry Cell culture Circulatory system biology.protein Azo Compounds Oxidation-Reduction |
| Zdroj: | Biochimica et biophysica acta. 885(1) |
| ISSN: | 0006-3002 |
| Popis: | Beating neonatal heart cell cultures were treated with diamide or t-butyl hydroperoxide, and changes in glutathione oxidation, cell beating, and protein S-thiolation (protein mixed-disulfide formation) were examined. Both compounds caused extensive oxidation of glutathione. Cells treated with diamide stopped beating within 2 min, and beating returned to normal after 30–45 min. Cells stopped beating 25 min after the addition of t-butyl hydroperoxide, and beating did not resume. t-Butyl hydroperoxide caused S-thiolation of a variety of proteins, but only one protein, of molecular mass 23 kDa, was extensively modified. Diamide caused extensive modification of proteins with molecular masses of 97, 42 and 23 kDa as well as three proteins of about 35 kDa. Though the GSSG content of cell cultures returned to normal by 15 min after diamide treatment. S-thiolation of several proteins persisted. These studies show that S-thiolation of proteins is an important metabolic response in cells exposed to an oxidative challenge by t-butyl hydroperoxide or diamide, and that the specificity of the response depends on the agent used. |
| Databáze: | OpenAIRE |
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