Overexpression of hippocampal Ca2+/calmodulin-dependent protein kinase II improves spatial memory
| Autor: | K. Spencer, Alex M. Babcock, Paul E. Micevych, D. Standing, K. Bullshields, David J. Poulsen |
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| Rok vydání: | 2006 |
| Předmět: |
Genetically modified mouse
Male Blotting Western Hippocampus Water maze Biology Hippocampal formation Motor Activity medicine.disease_cause Open field Gene Expression Regulation Enzymologic Viral vector Cellular and Molecular Neuroscience Memory Neuroplasticity medicine Reaction Time Animals Maze Learning Adeno-associated virus Behavior Animal Dependovirus Immunohistochemistry Rats Space Perception Calcium-Calmodulin-Dependent Protein Kinases Exploratory Behavior Calcium-Calmodulin-Dependent Protein Kinase Type 2 Neuroscience |
| Zdroj: | Journal of neuroscience research. 85(4) |
| ISSN: | 0360-4012 |
| Popis: | Hippocampal alpha-calcium/calmodulin-dependent protein kinase II (alphaCaMKII) has been implicated in neuronal plasticity and spatial learning. In the present experiment, an adeno-associated virus (AAV) vector was designed to express alphaCaMKII driven by the U6 promotor. Microinfusion of this vector into the rat hippocampus increased alphaCaMKII immunoreactivity by approximately 73% (Western analysis) and improved performance in a water maze task. Locomotor activity and exploratory behavior in an open field task were not altered by the overexpression of alphaCaMKII. These data support a role for alphaCaMKII in spatial or explicit memory storage. The advantages of viral vectors for manipulating target proteins expression compared with genetically modified mouse models are discussed. |
| Databáze: | OpenAIRE |
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