Differentiation of cardiomyocyte-like cells from human amniotic fluid mesenchymal stem cells by combined induction with human platelet lysate and 5-azacytidine
| Autor: | Waleephan Tancharoen, Sirinda Aungsuchawan, Suteera Narakornsak, Peraphan Pothacharoen, Runchana Markmee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell biology Amniotic fluid Molecular biology Immunofluorescence Regenerative medicine 03 medical and health sciences 0302 clinical medicine 5-azacytidine medicine Human platelet lysate Viability assay lcsh:Social sciences (General) Human amniotic fluid mesenchymal stem cells lcsh:Science (General) Multidisciplinary medicine.diagnostic_test GATA4 Chemistry Stem cell research Mesenchymal stem cell Cardiomyocyte-like cells Cardiomyogenic differentiation 030104 developmental biology Platelet lysate lcsh:H1-99 Biomedical engineering 030217 neurology & neurosurgery Research Article Differentiation Inducer lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 6, Iss 9, Pp e04844-(2020) Heliyon |
| ISSN: | 2405-8440 |
| Popis: | Human amniotic fluid mesenchymal stem cells (hAF-MSCs) have been shown to be effective in the treatment of many diseases. Platelet lysate (PL) contains multiple growth and differentiation factors; therefore, it can be used as a differentiation inducer. In this study, we attempted to evaluate the efficiency of human platelet lysate (hPL) on cell viability and the effects on cardiomyogenic differentiation of hAF-MSCs. When treating the cells with hPL, the result showed an increase in cell viability. Expressions of cardiomyogenic specific genes, including GATA4, cTnT, Cx43 and Nkx2.5, were higher in the combined treatment groups of 5-azacytidine (5-aza) and hPL than the expressions of cardiomyogenic specific genes in the control group and in the 5-aza treatment group. In terms of the results of immunofluorescence and immunoenzymatic staining, the highest expressions of cardiomyogenic specific proteins were revealed in combined treatment groups. It can be summarized that hPL may be an effective supporting cardiomyogenic supplementary factor for cardiomyogenic differentiation in hAF-MSCs. Cell biology; Biomedical engineering; Molecular biology; Regenerative medicine; Stem cell research; Cardiomyogenic differentiation; Cardiomyocyte-like cells; Human amniotic fluid mesenchymal stem cells; Human platelet lysate; 5-azacytidine. |
| Databáze: | OpenAIRE |
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