Ultrastructural visualization of Na+-channel associated[125]α-scorpion toxin binding sites on fetal mouse nerve cells in culture
| Autor: | J.L. Boudier, François Couraud, Jean-Louis Boudier, Annette Koulakoff, P. Cau, H.D. Dellmann, Yoheved Berwald-Netter |
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| Rok vydání: | 1985 |
| Předmět: |
Neurite
Androctonus australis Ion Channels Sodium Channels Mice Fetus Developmental Neuroscience Animals Neurotoxin Receptors Cholinergic Binding site Cell body membrane Receptor Neurons Binding Sites biology Sodium channel Cell Membrane Sodium Brain biology.organism_classification Molecular biology In vitro Microscopy Electron Autoradiography Developmental Biology |
| Zdroj: | Developmental Brain Research. 20:137-142 |
| ISSN: | 0165-3806 |
| Popis: | Purified neurotoxin II from the scorpion Androctonus australis Hector (alpha-ScTx) has previously been shown to bind specifically to the voltage-sensitive Na+ channels of excitable cells. Recent studies, using high specific activity 125I-labeled alpha-ScTx, demonstrated specific binding to neuronal cells derived from fetal mouse brains. In the present study, 125I-labeled alpha-ScTx was used to localize the voltage-sensitive Na+ channels in cultured fetal mouse brain cells. By quantitative electron microscope autoradiography we demonstrate that specific alpha-ScTx binding sites are selectively located at the plasma membrane. Estimates of their density revealed that neurites at 13 days in vitro carry at least 6 X more specific alpha-ScTx sites than cell body membrane. |
| Databáze: | OpenAIRE |
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