The gene-specific initiation factor USF (upstream stimulatory factor) bound at the adenovirus type 2 major late promoter: mass and three-dimensional structure
| Autor: | Robert G. Roeder, Michele Sawadogo, James F. Hainfeld, Iris A. Mastrangelo, Joseph S. Wall, Paul V. C. Hough |
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| Rok vydání: | 1987 |
| Předmět: |
Models
Molecular Multidisciplinary Base Sequence Genes Viral Protein Conformation Biology DNA-binding protein Molecular biology Upstream Stimulatory Factor Adenoviridae DNA-Binding Proteins Molecular Weight Viral Proteins Protein structure Transcription (biology) DNA Viral Microscopy Electron Scanning Nucleic Acid Conformation Initiation factor Promoter Regions Genetic Gene Transcription factor Research Article Transcription Factors Palindromic sequence |
| Zdroj: | Proceedings of the National Academy of Sciences. 84:4826-4830 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.84.14.4826 |
| Popis: | The gene-specific transcription initiation factor USF (upstream stimulatory factor) binds at a palindromic sequence that extends from -52 to -63 relative to the start site of the adenovirus type 2 major late promoter; USF enhances in vitro transcription 10- to 20-fold. By analysis of digital micrographs from the Brookhaven scanning transmission electron microscope, we have identified a sample of 29 proteins (mass, 55 +/- 5 kDa) specifically bound at the palindrome. The individual protein digital images show extensive homology, which permits modeling a three-dimensional structure at a relatively low resolution, which is nonetheless significant for the study of protein-protein interactions in initiation. Non-sequence-specific competitor DNA at high mass excess can be used in reactions for microscopy, enabling characterization of specific binding for proteins present at 1% of total protein or less. |
| Databáze: | OpenAIRE |
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