Identification of a novel marker for auxin and ethylene cross-talk from tobacco seedlings
| Autor: | Camille Roux, Ausra Dargeviciute, James Bauly, Catherine Perrot-Rechenmann |
|---|---|
| Přispěvatelé: | Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institute of Biotechnology |
| Rok vydání: | 2002 |
| Předmět: |
EXPRESSION
0106 biological sciences 1-Naphthaleneacetic acid Ethylene endocrine system diseases Physiology Nicotiana tabacum PROTEIN Plant Science urologic and male genital diseases 01 natural sciences 03 medical and health sciences chemistry.chemical_compound AUX/IAA GENE FAMILY Auxin Gene expression Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Transferase CELL-CULTURES Abscisic acid 030304 developmental biology RESPONSE PATHWAY chemistry.chemical_classification 0303 health sciences biology fungi food and beverages ABSCISIC-ACID biology.organism_classification TRANSPORT chemistry Biochemistry ARABIDOPSIS-THALIANA TOMATO MUTANT human activities Solanaceae ROOT GRAVITROPISM 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry Plant Physiology and Biochemistry, Elsevier, 2002, 40 (9), pp.803-811. ⟨10.1016/S0981-9428(02)01438-9⟩ |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/s0981-9428(02)01438-9 |
| Popis: | A novel early-response auxin-regulated gene, here termed Nt-AER (Auxin and Ethylene Regulated), was identified from a cDNA library prepared from auxin-treated tobacco (Nicotiana tabacum cv. Xanthi) seedlings. Comparison with sequence databases places Nt-AER in a family of transferase enzymes. Nt-AER was detected in a number of tobacco tissues with the exception of young leaves. In tobacco seedlings, Nt-AER is specifically up-regulated by auxins, but not their inactive analogues. A number of other plant growth regulators were tested of which the ethylene precursor ACC also induced Nt-AER accumulation. Steady-state levels of Nt-AER were relatively high in dark-grown tobacco seedlings but fell rapidly upon exposure to light and the possible role of ethylene in this response is discussed. Nt-AER was found to be rapidly induced following NAA or ACC treatment and maximum accumulation was detected for concentrations above 1 muM. Whilst ACC failed to induce Nt-AER in seedlings of transgenic ethylene insensitive tobacco, a response to NAA was retained. This suggests that Nt-AER is regulated by separable signalling pathways and not only by auxin-induced ethylene biosynthesis. As such, Nt-AER represents a novel marker for auxin and ethylene cross-talk. |
| Databáze: | OpenAIRE |
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