Sperm mRNAs and microRNAs as candidate markers for the impact of toxicants on human spermatogenesis: an application to tobacco smoking
| Autor: | Jeanne Perrin, Miriam Yammine, Cathy Nguyen, Geneviève Victorero, Pascal Rihet, Aurélie Bergon, Nicolas Boulanger, I. Sari-Minodier, Catherine Metzler-Guillemain, Cyrille Lepoivre |
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| Přispěvatelé: | Technologies avancées pour le génôme et la clinique (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Information génomique et structurale (IGS), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Université de Mons (UMons) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genetics
Male Messenger RNA education.field_of_study Urology Population Smoking Biology Sperm Spermatozoa Tobacco smoke 3. Good health Andrology MicroRNAs Reproductive Medicine microRNA Tobacco Humans RNA Messenger DNA microarray [SDE.BE]Environmental Sciences/Biodiversity and Ecology education Spermatogenesis Gene |
| Zdroj: | Systems Biology in Reproductive Medicine Systems Biology in Reproductive Medicine, 2015, 61 (3), pp.139--149. ⟨10.3109/19396368.2015.1022835⟩ |
| DOI: | 10.3109/19396368.2015.1022835⟩ |
| Popis: | International audience; Spermatozoa contain a complex population of RNAs including messenger RNAs (mRNAs) and small RNAs such as microRNAs (miRNA). It has been reported that these RNAs can be used to understand the mechanisms by which toxicological exposure affects spermatogenesis. The aim of our study was to compare mRNA and miRNA profiles in spermatozoa from eight smokers and eight non-smokers, and search for potential relationships between mRNA and miRNA variation. All men were selected based on their answers to a standard toxic exposure questionnaire, and sperm parameters. Using mRNA and miRNA microarrays, we showed that mRNAs from 15 genes were differentially represented between smokers and non-smokers (p \textless 0.01): five had higher levels and 10 lower levels in the smokers. For the microRNAs, 23 were differentially represented: 16 were higher and seven lower in the smokers (0.004 \textless= p \textless 0.01). Quantitative RT-PCR confirmed the lower levels in smokers compared to non-smokers for hsa-miR-296-5p, hsa-miR-3940, and hsa-miR-520d-3p. Moreover, we observed an inverse relationship between the levels of microRNAs and six potential target mRNAs (B3GAT3, HNRNPL, OASL, ODZ3, CNGB1, and PKD2). Our results indicate that alterations in the level of a small number of microRNAs in response to smoking may contribute to changes in mRNA expression in smokers. We conclude that large-scale analysis of spermatozoa RNAs can be used to help understand the mechanisms by which human spermatogenesis responds to toxic substances including those in tobacco smoke. |
| Databáze: | OpenAIRE |
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