Impedimetric paper-based biosensor for the detection of bacterial contamination in water
| Autor: | Saravanan Rengaraj, Janet L. Scott, Mirella Di Lorenzo, Álvaro Cruz-Izquierdo |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Microorganism
Nanotechnology 02 engineering and technology Lectin-bacteria complex 01 natural sciences Paper electronics SDG 3 - Good Health and Well-being Materials Chemistry Electrical and Electronic Engineering Instrumentation Detection limit biology Chemistry Screen printed electrode 010401 analytical chemistry Metals and Alloys Contamination Biodegradation 021001 nanoscience & nanotechnology biology.organism_classification Condensed Matter Physics 0104 chemical sciences Dielectric spectroscopy Electronic Optical and Magnetic Materials Surfaces Coatings and Films Electrode Screen printing 0210 nano-technology Electrochemical impedance spectroscopy Bacteria |
| Zdroj: | Rengaraj, S, Cruz-Izquierdo, A, Scott, J & Di Lorenzo, M 2018, ' Impedimetric paper-based biosensor for the detection of bacterial contamination in water ', Sensors and Actuators B: Chemical, vol. 265, pp. 50-58 . https://doi.org/10.1016/j.snb.2018.03.020 |
| Popis: | According to the World Health Organisation, worldwide waterborne diseases are responsible for nearly two million human deaths annually. Rapid and at-site screen of pathogenic microorganisms in drinking water can help to markedly reduce this number. Here we report an innovative, simple and low-cost, paper-based probe for detection of bacteria in water, fabricated by screen printing carbon electrodes onto hydrophobic paper. Electrochemical characterization of the printed electrodes confirmed fast-electron transfer, with an estimated electroactive surface area of 0.25 cm 2. The electrode surface was functionalised with carboxyl groups, prior to covalent immobilization of the lectin Concanavalin A (Con A), used as the biorecognition element. The system was then tested as an impedimetric sensor for bacteria in water. A linear increase in the probe charge transfer resistance was observed for bacterial concentrations ranging from 10 3 to 10 6 CFU mL −1, with an estimated lower detection limit of 1.9 × 10 3 CFU mL −1. Considering its remarkable simplicity, cost-effectiveness and biodegradability, the sensor here reported could be an attractive solution for portable testing kits that address the challenges of traditional time-consuming and expensive lab-based analyses. |
| Databáze: | OpenAIRE |
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