Anti-plasmodial and antioxidant activities of methanol extract of the fresh leaf of Lophira lanceolata (Ochnaceae)
| Autor: | Azubuike Onyeto Collins, Achunike Akah Peter, Sylvester Nworu Chukwuemeka, Chinwuba Okoye Theophine, null Nkemakolam, Nwakaego Mbaoji Florence, Amarachukwu Nwabunike Ifeoma, Okumah Nelson, Okpara Obinna |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Antioxidant Traditional medicine Chemistry DPPH medicine.medical_treatment Glycoside Applied Microbiology and Biotechnology Acute toxicity Terpenoid Reducing sugar chemistry.chemical_compound Phytochemical Biochemistry Genetics medicine Maceration (wine) Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 13, No 16 (2014) |
| ISSN: | 1684-5315 |
| Popis: | This study was aimed at investigating the anti-plasmodial and antioxidant activities of the extract of the leaf of Lophira lanceolata, a traditional medicine recipe. The methanol extract (ME) obtained by 72 h cold maceration was evaluated for acute toxicity test (LD50) and phytochemical constituents. The suppressive and curative anti-plasmodial activities of the extract were investigated using rodent malaria model. Mice (20 to 34 g) infected with 1 × 107 Plasmodium berghei parasitized red blood cell were used to test for suppressive and curative anti-plasmodial activities after oral administration of ME (100, 200 and 400 mg/kg) for four and seven days, respectively. The preliminary antioxidant activity of the extract (25, 50, 100, 200 and 400 μg/ml) was evaluated using in vitro 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, hydrogen peroxide scavenging and reducing (power) ability assays. The methanol leaf extract of L. lanceolata exhibited a dose-dependent suppression of parasitaemia up to 100% suppression at 400 mg/kg. The suppression produced by the extract was significantly (P< 0.05) higher than the chemo suppression produced by 20 mg/kg chloroquine (50.90%). Similarly, the extract at the same doses (100, 200 and 400 mg/kg) exhibited significant (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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