Syncytiotrophoblast micro-particles do not induce apoptosis in peripheral T lymphocytes, but differ in their activity depending on the mode of preparation
| Autor: | Corinne Rusterholz, Wolfgang Holzgreve, Anurag Kumar Gupta, Sinuhe Hahn |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cell Extracts
medicine.medical_specialty medicine.medical_treatment T cell Immunology Apoptosis Lymphocyte proliferation Biology Lymphocyte Activation Jurkat cells Immune system Syncytiotrophoblast Pre-Eclampsia Pregnancy Internal medicine medicine Immunology and Allergy Humans Cells Cultured Obstetrics and Gynecology T lymphocyte Cell biology Nanostructures Trophoblasts Endothelial stem cell Endocrinology medicine.anatomical_structure Cytokine Reproductive Medicine Female Chorionic Villi |
| Zdroj: | Journal of reproductive immunology. 68(1-2) |
| ISSN: | 0165-0378 |
| Popis: | Recent studies have suggested that pre-eclampsia may result from endothelial cell damage and overt immune activity triggered by the elevated release of syncytiotrophoblast-derived micro-particles (STBM). In this context, STBM have been reported to inhibit lymphocyte proliferation and induce Jurkat T cell apoptosis. In this study, STBM were prepared by three different in vitro methods (mechanical dissection, villous explant culture, and placental perfusion) and their functional properties were tested on T lymphocytes enriched from peripheral blood samples. Mechanically- and villous explant-derived STBM significantly inhibited activation, proliferation and cytokine release by T lymphocytes, while placental perfusion-derived STBM significantly induced T cell proliferation and a slight increase in IFNgamma release. None of the STBM preparations caused T cell apoptosis. Therefore, STBM prepared by different methods in vitro exhibit different effects on circulating T cells, a feature that will have to be taken into account when considering their potential role in normal pregnancy and pre-eclampsia. |
| Databáze: | OpenAIRE |
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