Detection of Acetyl Monoglyceride as a Metabolite of Newly Isolated Glycerol-assimilating Bacteria
| Autor: | Hiroyuki Iwabuchi, Tokuma Fukuoka, Takamitsu Tamura, Dai Kitamoto, Hiroshi Habe, Tomohiro Imura, Shingo Uemura, Keiji Sakaki, Tomotake Morita |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Glycerol
General Chemical Engineering Metabolite General Medicine General Chemistry Biology Monoglyceride biology.organism_classification 16S ribosomal RNA Enterobacteriaceae Amplified Ribosomal DNA Restriction Analysis Glycerides RNA Bacterial chemistry.chemical_compound chemistry Biochemistry RNA Ribosomal 16S Solvents Food Additives Ribosomal DNA Soil Microbiology Bacteria |
| Zdroj: | ResearcherID |
| ISSN: | 1347-3352 1345-8957 |
| DOI: | 10.5650/jos.58.147 |
| Popis: | Thirty-five glycerol-assimilating bacteria have recently been isolated from soil samples. Amplified ribosomal DNA restriction analysis revealed that these strains are grouped into four genetically different types of bacteria. Gas chromatography-mass spectrometry analysis of glycerol metabolites produced by the three selected strains (strains HH7, HH12, and HH31) revealed that extracts of culture liquid with ethyl acetate contains acetyl monoglyceride (monoacetin), which has not previously been reported as a glycerol metabolite and is used as a solvent, plasticizer, and food additive, as well as for other industrial purposes. The sequence analyses of the 16S rRNA genes from the selected strains showed that all of them belong to the Enterobacteriaceae. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|