Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques
Autor: | Hanping Liu, Xiaoyuan Deng, Qilei Zhang, Ning Wang, Maosheng Chen, Ying Jin, Xuefeng Li |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Genetically modified mouse Physiology Angiogenesis MSCs Bone Marrow Cells Mice Transgenic Vimentin Mesenchymal Stem Cell Transplantation lcsh:Physiology lcsh:Biochemistry Mice 03 medical and health sciences Paracrine signalling 0302 clinical medicine Animals Humans Regeneration lcsh:QD415-436 Cell Proliferation GFP-labeling Wound Healing lcsh:QP1-981 Tissue Engineering Tissue Scaffolds biology Chemistry Regeneration (biology) Mesenchymal stem cell ADM scaffolds Mesenchymal Stem Cells Actins Cell biology Transplantation 030104 developmental biology Cutaneous wound healing 030220 oncology & carcinogenesis Immunology biology.protein SHG-TPEF imaging Wound healing |
Zdroj: | Cellular Physiology and Biochemistry, Vol 42, Iss 2, Pp 623-639 (2017) |
ISSN: | 1421-9778 1015-8987 |
Popis: | Background/Aims: Bone marrow Mesenchymal stem cells (MSCs) are promising for promoting cutaneous wound healing through reinforcing cellular processes. We evaluated the effect of GFP-tagged MSCs transplantation on skin regeneration in excisional wounds in mice. Methods: MSCs from GFP-labeled transgenic mice were co-cultured with acellular dermal matrix (ADM) scaffolds, and MSC-ADM scaffolds were transplanted into surgical skin wounds of BALB/c mice. After implantation, the survival and behavior of MSCs were examined by second harmonic generation and two-photon excitation fluorescence imaging, western blotting and DNA amplification and sequencing. Results: GFP-tagged MSCs were retained inside the regenerating skin until day 14 post-transplantation. Alpha-smooth muscle actin (α-SMA) and vimentin (VIM) were detected at 3, 5, 7, and 14 days post-transplantation by immunofluorescence double labeling. Although the GFP+/α-SMA+- and GFP+/VIM+-cell numbers decreased gradually with healing time, α-SMA+- and VIM+-cell numbers significantly increased, most of them were endogenous functional cells which were related to angiogenesis and collagen fiber structural remodeling. Conclusion: Therefore, in the initial stage of wound healing, transplanted MSCs differentiated into functional cells and played paracrine roles to recruit more endogenous cells for tissue remodeling. With the disappearance of exogenous cells, endogenous cells were responsible for the latter stage of cutaneous wound healing. |
Databáze: | OpenAIRE |
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