Influence of column type and chromatographic conditions on the ion-exchange chromatography of immunoglobulins
| Autor: | Kervin Harrison, Yan-Bo Yang |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Swine
Ion chromatography Buffers Biochemistry Analytical Chemistry Mice chemistry.chemical_compound Affinity chromatography Spectrophotometry medicine Animals Ascitic Fluid Cation Exchange Resins Tromethamine Anion Exchange Resins Sheep Chromatography medicine.diagnostic_test Column temperature Chemistry Elution Osmolar Concentration Organic Chemistry Temperature Serum Albumin Bovine Blood Proteins General Medicine Buffer solution Hydrogen-Ion Concentration Chromatography Ion Exchange Rats Immunoglobulin G Cattle Spectrophotometry Ultraviolet Rabbits Leaching (metallurgy) Selectivity HEPES |
| Zdroj: | Journal of Chromatography A. 743:171-180 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/0021-9673(96)00255-5 |
| Popis: | Immunoglobulins are often purified by affinity chromatography. However, this technique is costly, can result in poor resolution for subclasses (or is only group specific), and leads to possible leaching of contaminants into the purified products. Ion-exchange chromatography has shown great potential and has found an increased usage in the purification of immunoglobulins. The aim of this study is to further understand the separation mechanism with emphasis on the influence of column type and chromatographic conditions on the peak shape, selectivity and changes in the elution patterns. Included are strong cation-exchange, strong anion-exchange and weak anion-exchange columns. Five immunoglobulin G antibodies were used as test probes. Some sera and ascites were also used in the study. Among the chromatographic conditions examined were mobile phase pH, buffer type, buffer concentration, gradient rate, and column temperature. Significant differences in the chromatographic behavior (elution pattern, peak shape and selectivity) of the test samples are discussed in regard to the column type and the chromatographic conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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