pH Sensitive Dextran Coated Fluorescent Nanodiamonds as a Biomarker for HeLa Cells Endocytic Pathway and Increased Cellular Uptake
| Autor: | Linyan Nie, Romana Schirhagl, Lei Li, Yue Zhang, Patrick van Rijn |
|---|---|
| Přispěvatelé: | Nanotechnology and Biophysics in Medicine (NANOBIOMED), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
General Chemical Engineering
Endocytic cycle PROTEIN Endocytosis Pathway 02 engineering and technology Article HeLa 03 medical and health sciences chemistry.chemical_compound endocytic pathway LYSOSOME Lysosome medicine NANOPARTICLES General Materials Science DRUG-DELIVERY Nanodiamond QD1-999 030304 developmental biology 0303 health sciences biology Chemistry cellular uptake 021001 nanoscience & nanotechnology biology.organism_classification Fluorescence DYSFUNCTION medicine.anatomical_structure Dextran nanodiamonds Biophysics 0210 nano-technology Intracellular ACIDIFICATION |
| Zdroj: | Nanomaterials, 11(7):1837, 1-11. MDPI AG Nanomaterials Volume 11 Issue 7 Nanomaterials, Vol 11, Iss 1837, p 1837 (2021) |
| ISSN: | 2079-4991 |
| Popis: | Fluorescent nanodiamonds are a useful for biosensing of intracellular signaling networks or environmental changes (such as temperature, pH or free radical generation). HeLa cells are interesting to study with these nanodiamonds since they are a model cell system that is widely used to study cancer-related diseases. However, they only internalize low numbers of nanodiamond particles very slowly via the endocytosis pathway. In this work, we show that pH-sensitive, dextran-coated fluorescent nanodiamonds can be used to visualise this pathway. Additionally, this coating improved diamond uptake in HeLa cells by 5.3 times (*** p < 0.0001) and decreased the required time for uptake to only 30 min. We demonstrated further that nanodiamonds enter HeLa cells via endolysosomes and are eventually expelled by cells. |
| Databáze: | OpenAIRE |
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