Biosafety evaluation of recombinant protein production in goat mammary gland using adenoviral vectors: Preliminary study
| Autor: | Pastor Alfonso Zamora, Elaine Santana Rodríguez, Alaín González Pose, María Pilar Rodríguez Moltó, Marisela Suárez Pedroso, Angela Sosa Espinoza |
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| Rok vydání: | 2012 |
| Předmět: |
Saliva
Genetic Vectors Mammary gland Antibodies Viral Transfection Applied Microbiology and Biotechnology Adenoviridae law.invention Animals Genetically Modified Biosafety Transduction (genetics) Mammary Glands Animal law Propiolactone medicine Animals Analysis of Variance biology Goats General Medicine Virology Recombinant Proteins Genetically modified organism Methylene Blue Milk medicine.anatomical_structure Recombinant DNA biology.protein Virus Inactivation Molecular Medicine Female Antibody Biotechnology |
| Zdroj: | Biotechnology Journal. 7:1049-1053 |
| ISSN: | 1860-6768 |
| Popis: | The production of recombinant proteins in the milk of non-transgenic goats can be achieved by transducing the mammary gland with recombinant adenoviral vectors. However, this process involves several regulatory issues. The current study evaluates the biosafety of this production system. We present a preliminary biosafety profile based on detection of adenoviral particles in different body fluids and the antibody response after adenoviral transduction of the goat mammary gland. In addition, two methods of adenoviral inactivation in milk were tested. Although adenoviral particles were detected in the milk until day 4 after transduction, they were absent in serum, saliva, urine and feces. Anti-adenovirus antibodies were detected in serum and milk. The virus inactivation methods neutralized adenoviral particles and preserved the immunological identity of the recombinant protein. These results support the idea of a safe production of recombinant proteins using adenoviral vectors. |
| Databáze: | OpenAIRE |
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