Immunocytochemical detection of bone marrow micrometastases in colorectal carcinoma patients, using a monoclonal antibody to villin
| Autor: | Frédéric Grozier, Sylvie Robine, Anne Berger, Roman Rouzier, E. Bourstyn, Daniel Louvard |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Pathology medicine.medical_specialty Colorectal cancer medicine.drug_class Immunocytochemistry Biophysics Bone Marrow Cells Monoclonal antibody Sensitivity and Specificity Pathology and Forensic Medicine Cytokeratin Endocrinology Biomarkers Tumor Tumor Cells Cultured medicine Humans Aged Aged 80 and over biology Carcinoma Microfilament Proteins Antibodies Monoclonal Cell Biology Hematology Middle Aged medicine.disease Immunohistochemistry Molecular biology medicine.anatomical_structure Cancer cell biology.protein Keratins Female Bone marrow Antibody Bone Marrow Neoplasms Carrier Proteins Colorectal Neoplasms Villin |
| Zdroj: | Cytometry. 46:281-289 |
| ISSN: | 1097-0320 0196-4763 |
| Popis: | The search continues to find methods to more effectively distinguish colorectal carcinoma patients who could be separated into high-risk and low-risk categories. Investigators have reported on the detection of occult micrometastases in bone marrow using antibodies to cytokeratin, which is a marker of epithelial cells but which has no tissue specificity, as opposed to villin, a cytoskeletal protein that is specifically involved in the formation of brush-border microvilli in the small intestine and colon epithelium. Specificity and sensitivity of antibody to villin (ID2C3) and antibody to cytokeratin (A45-B/B3) were first studied in normal bone marrow and in a test system in which cancer cell lines were mixed in normal bone marrow. In a preliminary study including 16 colorectal carcinoma patients, we compared the number of villin-positive cells with cytokeratin-presenting cells. As A45-B/B3, ID2C3 was determined to be sensitive enough to detect one cancer cell in 106 hematopoietic cells. Staining of hematopoietic cells with irrelevant antibody and a light staining of megakaryocytes with ID2C3 limited the specificity of the method. In colorectal carcinoma patients, correlation between ID2C3 and A45-B/B3 was 94%. Sensitivity and specificity of ID2C3 antibody to villin were satisfactory. Its clinical relevance must be investigated in further studies. Cytometry (Comm. Clin. Cytometry) 46:281–289, 2001. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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