Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region
| Autor: | Diego Garzón-Ospina, Manuel A. Patarroyo, Juan Ricardo Cubides, Paola Andrea Camargo-Ayala, María Fernanda Orduz-Durán, Manuel E. Patarroyo, Carlos Hernando Niño, Estefany Ospina-Cantillo, Anggie Ortega-Ortegón |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene Mutation Plasmodium Plasmodium vivax Drug Resistance Protozoan Proteins DHPS Gene mutation Gene Polymerase Chain Reaction 0302 clinical medicine Dhps Gene Vacuna contra la malaria Haplotype Plasmodium Vivax Antimalarial drug resistance Crt O Gene Plasmodium Vivax Malaria Genetics education.field_of_study biology Malaria vaccine Mdr1 Gene Infectious Diseases Genetic Variability Dhfr Gene Crt O génica Malaria Control pvdhfr Genetic Association Human Electrophoresis Gene Sequence lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 030231 tropical medicine 030106 microbiology Population Endemic Disease Disease Surveillance Genetic Resistance Colombia Article lcsh:Infectious and parasitic diseases 03 medical and health sciences Antimalarials Malaria Vaccine Gene Location parasitic diseases medicine lcsh:RC109-216 Amazonian region education Polymorphism Genetic Research Nucleotide Sequence biology.organism_classification medicine.disease Nonhuman Enfermedades Malaria pvmdr1 and pvcrt-o Haplotypes pvdhps Mutation Genetic Polymorphism Parasitology Genetic Selection Selective sweep |
| Zdroj: | Malaria Journal, Vol 17, Iss 1, Pp 1-9 (2018) Malaria Journal (2016) World Malaria Report 2016, WHO World Health Organization Geneva Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario |
| ISSN: | 1475-2875 |
| DOI: | 10.1186/s12936-018-2286-5 |
| Popis: | Background: Malaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region. Methods: Fifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained. Conclusion: pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage. © 2018 The Author(s). |
| Databáze: | OpenAIRE |
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