HPLC analysis of the second-generation antidepressant sertraline and its main metabolite N-desmethylsertraline in human plasma
| Autor: | Maria Addolorata Saracino, Domenico Berardi, Maria Augusta Raggi, Silvia Ferrari, Ernst Kenndler, Roberto Mandrioli |
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| Přispěvatelé: | R. Mandrioli, M.A. Saracino, S. Ferrari, D. Berardi, E. Kenndler, M.A. Raggi |
| Rok vydání: | 2006 |
| Předmět: |
Analyte
Metabolite Clinical Biochemistry SOLID PHASE EXTRACTION Sensitivity and Specificity Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Sertraline N-DESMETHYLSERTRALINE Humans HUMAN PLASMA Solid phase extraction Acetonitrile Triethylamine Chromatography High Pressure Liquid Chromatography Extraction (chemistry) Reproducibility of Results Cell Biology General Medicine LIQUID CHROMATOGRAPHY chemistry Yield (chemistry) Selective Serotonin Reuptake Inhibitors |
| Zdroj: | Journal of Chromatography B. 836:116-119 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2006.03.026 |
| Popis: | A liquid chromatographic method with ultraviolet detection was developed for the analysis of the recent antidepressant sertraline and its main metabolite N-desmethylsertraline in human plasma. The analytes were separated on a C8 reversed phase column, using a mobile phase composed of acetonitrile and a 12.3 mM, pH 3.0 phosphate buffer containing 0.1% triethylamine (35:65, v/v). Clomipramine was used as the Internal Standard. Using a solid phase extraction procedure with C2 cartridges high extraction yields (94%) and good purification from matrix interference were obtained. Good linearity was obtained in the 7.5-250.0 ng mL(-1) range for sertraline and in the 10-500 ng mL(-1) range for N-desmethylsertraline. The analytical method was validated in terms of precision, extraction yield and accuracy. These assays gave R.S.D.% values for precision always lower than 3.9% and mean accuracy higher than 90%. Thanks to its good selectivity, the method proved to be suitable for the analysis of plasma samples from patients treated with sertraline as either monotherapy or polypharmacy. |
| Databáze: | OpenAIRE |
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