Quantitative measurement of HER2 expression in breast cancers: comparison with ‘real-world’ routine HER2 testing in a multicenter Collaborative Biomarker Study and correlation with overall survival
| Autor: | Jodi Weidler, Lea Krekow, Rick L. Pesano, Stephen Zrada, Jeff Sperinde, A. Paquet, Marlon Brigino, Steve Anderson, John W Adams, Mojgan Haddad, Peter A. Kaufman, Yolanda Lie, Lee S. Schwartzberg, Harvey Einhorn, Michael Savin, Weidong Huang, Alexander Starr, William E Lawler, Linda D. Bosserman, Michael Bates, Denise A. Yardley |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Oncology medicine.medical_specialty Receptor ErbB-2 Concordance Breast Neoplasms Kaplan-Meier Estimate Young Adult Breast cancer Risk Factors Surgical oncology Internal medicine Biomarkers Tumor medicine Humans skin and connective tissue diseases neoplasms In Situ Hybridization Fluorescence Aged Neoplasm Staging Retrospective Studies Medicine(all) Gynecology medicine.diagnostic_test business.industry Reproducibility of Results Retrospective cohort study Middle Aged Prognosis medicine.disease Immunohistochemistry Tumor Burden Cohort Biomarker (medicine) Female Neoplasm Grading business Follow-Up Studies Research Article Fluorescence in situ hybridization |
| Zdroj: | Breast Cancer Research : BCR |
| ISSN: | 1465-542X |
| DOI: | 10.1186/s13058-015-0543-x |
| Popis: | Introduction Accurate assessment of HER2 status is critical in determining appropriate therapy for breast cancer patients but the best HER2 testing methodology has yet to be defined. In this study, we compared quantitative HER2 expression by the HERmark™ Breast Cancer Assay (HERmark) with routine HER2 testing by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), and correlated HER2 results with overall survival (OS) of breast cancer patients in a multicenter Collaborative Biomarker Study (CBS). Methods Two hundred and thirty-two formalin-fixed, paraffin-embedded breast cancer tissues and local laboratory HER2 testing results were provided by 11 CBS sites. HERmark assay and central laboratory HER2 IHC retesting were retrospectively performed in a blinded fashion. HER2 results by all testing methods were obtained in 192 cases. Results HERmark yielded a continuum of total HER2 expression (H2T) ranging from 0.3 to 403 RF/mm2 (approximately 3 logs). The distribution of H2T levels correlated significantly (P |
| Databáze: | OpenAIRE |
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