Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells
| Autor: | Jose Javier Bravo-Cordero, Louis Hodgson, Yasmin Moshfegh, John S. Condeelis |
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| Rok vydání: | 2013 |
| Předmět: |
rho GTP-Binding Proteins
Biosensing Techniques macromolecular substances Cell Surface Extension Matrix (biology) Biology Actin cytoskeleton Article Extracellular Matrix Molecular Imaging Cell biology Extracellular matrix Actin Cytoskeleton Cell Movement Live cell imaging Cell Line Tumor Neoplasms Invadopodia Humans Cell Surface Extensions Signal transduction Molecular Biology Extracellular Matrix Degradation |
| Zdroj: | Adhesion Protein Protocols ISBN: 9781627035378 |
| DOI: | 10.1007/978-1-62703-538-5_22 |
| Popis: | Tumor cell motility and invasion rely on actin cytoskeleton rearrangements mediated by the activation of RhoGTPase signaling pathways. Invadopodia are membrane-degrading protrusions that mediate extracellular matrix degradation. Here, we provide procedures for imaging RhoGTPase biosensors in tumor cells during the formation of invadopodia and matrix degradation. |
| Databáze: | OpenAIRE |
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