Use of enzyme-linked synthetic DNA in diagnosis of falciparum malaria
| Autor: | GeraldL. Mclaughlin, Edward G. Jablonski, JerryL. Ruth, Richard W. Steketee, GaryH. Campbell |
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| Rok vydání: | 1987 |
| Předmět: |
Plasmodium falciparum
Antigens Protozoan Biology Genome Nucleic acid thermodynamics chemistry.chemical_compound parasitic diseases Humans Repeated sequence chemistry.chemical_classification Nucleic Acid Hybridization General Medicine DNA biology.organism_classification Alkaline Phosphatase Virology Molecular biology Kenya Malaria Enzyme chemistry Alkaline phosphatase Protozoa Female Carrier Proteins |
| Zdroj: | Lancet (London, England). 1(8535) |
| ISSN: | 0140-6736 |
| Popis: | Plasmodium falciparum contains a family of 21-base-long repetitive DNA sequences in its genome. A 21-base synthetic DNA oligomer, formerly labelled with phosphorus-32 for autoradiographic detection of P falciparum DNA, was covalently coupled to alkaline phosphatase for histochemical detection. The conjugate (PFR1-AP) detected purified P falciparum DNA with a sensitivity and specificity equal to that of 32P-labelled probes after 2-day exposures. PFR1-AP did not detect host DNA or DNA of other Plasmodium species. In African blood specimens PFR1-AP specifically detected P falciparum infections of 100 parasites/microliter. This sensitive, rapid, nonisotopic probe will allow more widespread use of DNA hybridisation in the diagnosis of malaria. |
| Databáze: | OpenAIRE |
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