A Method for Histological Preparation of Undecalcified Bone Sections Containing Acrylic Bone Cement
Autor: | Lars Nimb Jensen, Klaus Gotfredsen, Jørgen Steen Jensen |
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Rok vydání: | 1991 |
Předmět: |
Histology
Materials science Dentistry Bone and Bones Acrylic Bone Cement Dogs Animals Methylmethacrylates Hematoxylin Staining and Labeling Bone decalcification Histocytochemistry business.industry Bone Cements technology industry and agriculture General Medicine Epoxy equipment and supplies Bone cement Medical Laboratory Technology Undecalcified bone visual_art visual_art.visual_art_medium Eosine Yellowish-(YS) business |
Zdroj: | University of Copenhagen |
ISSN: | 1473-7760 1052-0295 |
Popis: | An improved and time reducing method is presented for the histological evaluation of bone containing polymethylmethacrylate (PMMA) bone cement. The undecalcified bone was embedded in epoxy resin and section of 50-100 microns thickness were produced using a commercially available cutting grinding system. The sections were stained with Stevenel's blue and van Gieson picrofuchsin or a modified hematoxylineosin. PMMA bone cement was not dissolved and remained enabling examination in situ of an intact cement bone interface and tissue reaction without decalcification. |
Databáze: | OpenAIRE |
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