A Method for Histological Preparation of Undecalcified Bone Sections Containing Acrylic Bone Cement

Autor: Lars Nimb Jensen, Klaus Gotfredsen, Jørgen Steen Jensen
Rok vydání: 1991
Předmět:
Zdroj: University of Copenhagen
ISSN: 1473-7760
1052-0295
Popis: An improved and time reducing method is presented for the histological evaluation of bone containing polymethylmethacrylate (PMMA) bone cement. The undecalcified bone was embedded in epoxy resin and section of 50-100 microns thickness were produced using a commercially available cutting grinding system. The sections were stained with Stevenel's blue and van Gieson picrofuchsin or a modified hematoxylineosin. PMMA bone cement was not dissolved and remained enabling examination in situ of an intact cement bone interface and tissue reaction without decalcification.
Databáze: OpenAIRE