Effects of Combined Treatment with Vitamin D and COX2 Inhibitors on Breast Cancer Cell Lines
| Autor: | Kathrin Reichert, Michael W. Friedrich, Friederike Hoellen, Alena Woeste, Dorothea Fischer, S. Polack, Marc Thill, Frank Köster, Achim Rody |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Calcitriol Apoptosis Breast Neoplasms macromolecular substances 03 medical and health sciences Western blot Tumor Cells Cultured medicine Vitamin D and neurology Humans Prostaglandin E2 skin and connective tissue diseases Cell Proliferation Cyclooxygenase 2 Inhibitors medicine.diagnostic_test Cell growth Chemistry food and beverages Vitamins General Medicine Metabolism 030104 developmental biology Oncology Celecoxib Cell culture Cancer research Drug Therapy Combination Female lipids (amino acids peptides and proteins) medicine.drug |
| Zdroj: | Anticancer Research. 38 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.12340 |
| Popis: | Background Vitamin D is known for its anticancer potential. Prostaglandin E2 (PGE2) is a proliferative and inflammation-activating agent. The production of PGE2 is dependent on the activity of cyclooxygenase-2 (COX2). A link between vitamin D and PGE2 metabolism was shown recently. Materials and methods In MDA-MB-231 and MCF-7 breast cancer cell lines, we investigated the influence of calcitriol and the COX2 inhibitor celecoxib on cell growth via the MTT test, as well as on the protein and mRNA expression of COX2 using western blot and quantitative real-time polymerase chain reaction (qRT-PCR). Results The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX2 inhibitor celecoxib and even more strongly by their combination. Moreover, calcitriol inhibited COX2 protein expression in MDA-MB-231 cells, as well as COX2 mRNA expression in both cell lines. Conclusion The combination of calcitriol and celecoxib demonstrated a synergistic growth-inhibitory effect in breast cancer cell lines. |
| Databáze: | OpenAIRE |
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